| Id |
Target |
Expression Suffix |
Clone |
Local Clone |
Local Protein Target |
Local Expression |
Experiment Date Start |
Experiment Date End |
Staff |
Lab |
Protocol |
Status |
Expression Organism |
Expression Strain |
Media Type |
Media Volume |
Growth Temperature |
Induction Temperature |
Induction Reagent |
Experiment Type |
Expression Level |
Solubility Level |
Actions |
|
11047 |
IDP01880 |
31 |
7437 |
|
|
|
Sep 13, 2009 |
|
David Cooper |
COLLABORATORS |
standard expression_ANL |
success |
E. coli |
BL21(DE3) |
TB |
2400 |
37 |
20 |
1 mM IPTG |
production |
|
|
View Edit Delete |
|
7293 |
IDP90271 |
2 |
5556 |
|
|
|
Oct 15, 2009 |
Oct 16, 2009 |
Chris Nelson |
WU |
WU_native_soluble_expression_1 |
success |
E. coli |
BL21-CodonPlus(DE3)-RIL |
LB |
1000 |
30 |
25 |
0.5 IPTG |
production |
50 |
50 |
View Edit Delete |
|
3485 |
IDP00007 |
10 |
1783 |
|
|
|
Jul 03, 2008 |
Jul 03, 2008 |
Olena Onopriyenko |
UT |
Expression_protocol_Toronto |
success |
E. coli |
BL21-CodonPlus(DE3)-RIPL |
Se-Met |
1 |
37 |
20 |
none |
production |
|
|
View Edit Delete |
|
4690 |
IDP00947 |
1 |
2631 |
STM0988P7 |
|
|
Oct 20, 2008 |
Dec 02, 2008 |
Charitha Nakka |
UTSW |
UTSW_standard expression |
success |
E.coli |
Rosetta(DE3)pLysS |
TB |
2 |
37 |
20 |
IPTG |
production |
|
|
View Edit Delete |
|
7294 |
IDP90271 |
3 |
5557 |
|
|
|
Oct 15, 2009 |
Oct 16, 2009 |
Chris Nelson |
WU |
WU_native_soluble_expression_1 |
success |
E. coli |
BL21-CodonPlus(DE3)-RIL |
LB |
1000 |
30 |
25 |
0.5 IPTG |
production |
70 |
70 |
View Edit Delete |
|
8809 |
IDP90521 |
1 |
5709 |
|
|
|
Nov 10, 2009 |
|
Charitha Nakka |
UTSW |
UTSW_standard expression |
success |
E.coli |
Rosetta(DE3)pLysS |
TB |
2 |
37 |
20 |
1 mM IPTG |
production |
|
|
View Edit Delete |
|
4691 |
IDP00947 |
2 |
2033 |
STM0988p19C |
|
|
Oct 20, 2008 |
Dec 05, 2008 |
Charitha Nakka |
UTSW |
UTSW_standard expression |
failed |
E.coli |
Rosetta(DE3)pLysS |
TB |
2 |
37 |
20 |
IPTG |
production |
|
|
View Edit Delete |
|
7282 |
IDP02119 |
1 |
5143 |
|
|
|
Sep 22, 2009 |
|
Charitha Nakka |
UTSW |
UTSW_standard expression |
success |
E.coli |
Rosetta(DE3)pLysS |
TB |
1 |
37 |
20 |
1mM IPTG |
production |
|
|
View Edit Delete |
|
18516 |
IDP91762 |
111 |
21446 |
|
|
|
Nov 04, 2012 |
Nov 07, 2012 |
Boguslaw Nocek |
ANL |
standard expression_ANL |
success |
E. coli |
BL21(DE3)magic |
M9 minimal |
5 |
37 |
18 |
1 mM IPTG |
assay |
90 |
90 |
View Edit Delete |
|
7283 |
IDP02668 |
1 |
4430 |
|
|
|
Mar 04, 2009 |
|
Charitha Nakka |
UTSW |
UTSW_standard expression |
success |
E.coli |
Rosetta(DE3)pLysS |
TB |
2 |
37 |
20 |
1 mM IPTG |
production |
|
|
View Edit Delete |
|
1800 |
IDP00007 |
4 |
1653 |
|
|
J0001226 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
4144 |
IDP00044 |
4 |
1685 |
|
|
J0001354 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
20 |
100 |
View Edit Delete |
|
7290 |
IDP90271 |
1 |
5555 |
|
|
IDP90271.301.1 |
Oct 15, 2009 |
Oct 16, 2009 |
Chris Nelson |
WU |
WU_native_soluble_expression_1 |
success |
E. coli |
BL21-CodonPlus(DE3)-RIL |
LB |
10000 |
30 |
25 |
0.5 mM IPTG |
production |
10 |
10 |
View Edit Delete |
|
13295 |
IDP00563 |
2161 |
6907 |
2660 |
+01B95YakdqYxalnNbktgrTkIok |
2161 |
Jul 06, 2010 |
Jul 08, 2010 |
Magdalena Makowska-Grzyska |
ANL |
standard expression_ANL |
success |
E. coli |
BL21(DE3)magic |
M9 minimal |
3000 |
37 |
16 |
0.5 mM IPTG |
production |
30 |
60 |
View Edit Delete |
|
13296 |
IDP00563 |
1372 |
6907 |
2660 |
+01B95YakdqYxalnNbktgrTkIok |
1372 |
Jun 08, 2010 |
Jun 10, 2010 |
Magdalena Makowska-Grzyska |
ANL |
standard expression_ANL |
success |
E. coli |
BL21(DE3)magic |
M9 minimal |
1000 |
37 |
16 |
0.5 mM IPTG |
production |
30 |
80 |
View Edit Delete |
|
13469 |
IDP04432 |
1672 |
6381 |
2802 |
02BuioSjXSQsfAxVmZf1CPFtGLk |
1672 |
Feb 02, 2010 |
Feb 04, 2010 |
Natalia Maltseva |
ANL |
standard expression_ANL |
success |
E. coli |
BL21(DE3)magic |
M9 minimal + semet |
1000 |
37 |
16 |
0.5 mM IPTG |
production |
50 |
50 |
View Edit Delete |
|
13468 |
IDP04432 |
1681 |
6381 |
2802 |
02BuioSjXSQsfAxVmZf1CPFtGLk |
1681 |
Mar 02, 2010 |
Feb 04, 2010 |
Natalia Maltseva |
ANL |
standard expression_ANL |
success |
E. coli |
BL21(DE3)magic |
M9 minimal + semet |
2000 |
37 |
16 |
0.5 mM IPTG |
production |
50 |
50 |
View Edit Delete |
|
16020 |
IDP00418 |
1302 |
285 |
1637 |
038c6EygZ+qtq8nW7WIAgL1J9R8 |
1302 |
Oct 07, 2008 |
Oct 09, 2008 |
Natalia Maltseva |
ANL |
standard expression_ANL |
success |
E. coli |
BL21(DE3)magic |
M9 minimal + semet |
1000 |
37 |
18 |
0.5 mM IPTG |
production |
100 |
100 |
View Edit Delete |
|
12785 |
IDP00933 |
5343000 |
9144 |
5343000 |
040Y3Ng5HH2pY1Zy97uWVogCf9g |
5343000 |
Feb 25, 2010 |
Mar 11, 2010 |
Minyi Gu |
ANL |
standard expression_ANL |
success |
E. coli |
BL21(DE3)magic |
M9 minimal |
1 |
37 |
18 |
1 mM IPTG |
assay |
80 |
20 |
View Edit Delete |
|
13098 |
IDP01468 |
1255 |
2303 |
1610 |
0auRkrouZMOlsCuhiLCzJYMEW5Y |
1255 |
Nov 05, 2008 |
Nov 08, 2008 |
Min Zhou |
ANL |
standard expression_ANL |
success |
E. coli |
BL21(DE3)magic |
M9 minimal + semet |
2000 |
37 |
16 |
1 mM IPTG |
production |
100 |
0 |
View Edit Delete |