| Id |
Target |
Expression Suffix |
Clone |
Local Clone |
Local Protein Target |
Local Expression |
Experiment Date Start |
Experiment Date End |
Staff |
Lab |
Protocol |
Status |
Expression Organism |
Expression Strain |
Media Type |
Media Volume |
Growth Temperature |
Induction Temperature |
Induction Reagent |
Experiment Type |
Expression Level |
Solubility Level |
Actions |
|
1726 |
IDP00152 |
3 |
1838 |
|
|
J0001447 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
100 |
View Edit Delete |
|
1849 |
IDP00064 |
4 |
1702 |
|
|
J0001422 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1869 |
IDP00163 |
6 |
1722 |
|
|
J0001502 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1841 |
IDP00054 |
4 |
1694 |
|
|
J0001390 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1581 |
IDP00054 |
3 |
1694 |
|
|
J0001389 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1615 |
IDP00144 |
3 |
1728 |
|
|
J0001525 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
10 |
0 |
View Edit Delete |
|
1607 |
IDP00161 |
5 |
1720 |
|
|
J0001493 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
15 |
0 |
View Edit Delete |
|
1863 |
IDP00157 |
6 |
1716 |
|
|
J0001478 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1862 |
IDP00156 |
6 |
1715 |
|
|
J0001474 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1645 |
IDP00180 |
5 |
1758 |
|
|
J0001645 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1734 |
IDP00158 |
3 |
1846 |
|
|
J0001479 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1577 |
IDP00049 |
3 |
1690 |
|
|
J0001373 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1967 |
IDP00049 |
2 |
1819 |
|
|
J0001372 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
5 |
100 |
View Edit Delete |
|
2020 |
IDP00188 |
4 |
1872 |
|
|
J0001584 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1887 |
IDP00193 |
6 |
1740 |
|
|
J0001574 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1707 |
IDP00049 |
1 |
1819 |
|
|
J0001371 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1816 |
IDP00025 |
4 |
1669 |
|
|
J0001290 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
15 |
33 |
View Edit Delete |
|
1881 |
IDP00153 |
4 |
1734 |
|
|
J0001550 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1582 |
IDP00055 |
3 |
1695 |
|
|
J0001393 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1861 |
IDP00155 |
6 |
1714 |
|
|
J0001470 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |