| Id |
Target |
Expression Suffix |
Clone |
Local Clone |
Local Protein Target |
Local Expression |
Experiment Date Start |
Experiment Date End |
Staff |
Lab |
Protocol |
Status |
Expression Organism |
Expression Strain |
Media Type |
Media Volume |
Growth Temperature |
Induction Temperature |
Induction Reagent |
Experiment Type |
Expression Level |
Solubility Level |
Actions |
|
7378 |
IDP00137 |
1 |
4650 |
|
|
|
Apr 29, 2009 |
Apr 30, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
25 |
100 |
View Edit Delete |
|
4471 |
IDP00044 |
168 |
1434 |
9 |
595 |
168 |
Feb 13, 2009 |
Feb 14, 2009 |
Majka Klimecka |
UVA |
uva_expression_semet_1 |
success |
E.coli |
E.coli BL21 DE3 Codon Plus RIPL |
SeMet |
4000 |
37 |
20 |
IPTG |
production |
80 |
80 |
View Edit Delete |
|
7211 |
IDP90224 |
31526 |
5463 |
|
|
|
Apr 13, 2007 |
|
Olga Kagan |
UT |
Expression_protocol_Toronto |
success |
E. coli |
BL21-CodonPlus(DE3)-RP |
Se-Met |
1 |
37 |
20 |
none |
production |
0 |
20 |
View Edit Delete |
|
9307 |
IDP04652 |
1 |
6535 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
100 |
View Edit Delete |
|
9306 |
IDP04651 |
1 |
6534 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
6970 |
IDP02676 |
43068 |
4436 |
|
|
|
May 15, 2009 |
|
Elena Gordon |
UT |
Expression_protocol_Toronto |
success |
E. coli |
BL21-CodonPlus(DE3)-RIPL |
Se-Met |
1 |
37 |
20 |
none |
production |
100 |
100 |
View Edit Delete |
|
2693 |
IDP01155 |
2 |
2360 |
|
|
J0002434 |
Sep 16, 2008 |
Sep 17, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9294 |
IDP04639 |
1 |
6522 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
100 |
View Edit Delete |
|
9308 |
IDP04653 |
1 |
6536 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
10 |
0 |
View Edit Delete |
|
1645 |
IDP00180 |
5 |
1758 |
|
|
J0001645 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
5766 |
IDP00901 |
3 |
2575 |
|
|
|
Oct 13, 2008 |
Oct 14, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
5767 |
IDP00901 |
4 |
1977 |
|
|
|
Oct 14, 2008 |
Oct 15, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
13682 |
IDP05232 |
665 |
8254 |
655 |
582 |
665 |
Aug 03, 2011 |
Aug 03, 2011 |
James Winsor |
NU |
NU_native_expression |
success |
E. coli |
KRX |
TB |
2000 |
37 |
37 |
IPTG |
production |
|
|
View Edit Delete |
|
13683 |
IDP91172 |
668 |
9283 |
658 |
465 |
668 |
Aug 17, 2011 |
Aug 17, 2011 |
James Winsor |
NU |
NU_native_expression |
success |
E. coli |
Rosetta |
TB |
1500 |
37 |
37 |
IPTG |
production |
|
|
View Edit Delete |
|
13684 |
IDP91172 |
669 |
9284 |
659 |
465 |
669 |
Aug 17, 2011 |
Aug 17, 2011 |
James Winsor |
NU |
NU_native_expression |
success |
E. coli |
Rosetta |
TB |
1500 |
37 |
37 |
IPTG |
production |
|
|
View Edit Delete |
|
13685 |
IDP91162 |
670 |
8017 |
512 |
386 |
670 |
Aug 17, 2011 |
Aug 17, 2011 |
James Winsor |
NU |
NU_native_expression |
success |
E. coli |
BL21 |
TB |
1500 |
37 |
37 |
IPTG |
production |
|
|
View Edit Delete |
|
13686 |
IDP90819 |
671 |
5990 |
343 |
295 |
671 |
Aug 17, 2011 |
Aug 17, 2011 |
James Winsor |
NU |
NU_native_expression |
success |
E. coli |
BL21 |
TB |
1500 |
37 |
37 |
IPTG |
production |
|
|
View Edit Delete |
|
13687 |
IDP90924 |
672 |
6079 |
351 |
302 |
672 |
Aug 17, 2011 |
Aug 17, 2011 |
James Winsor |
NU |
NU_native_expression |
success |
E. coli |
BL21 |
TB |
1500 |
37 |
37 |
IPTG |
production |
|
|
View Edit Delete |
|
9293 |
IDP04638 |
1 |
6521 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
0 |
View Edit Delete |
|
8235 |
IDP02663 |
47618 |
4858 |
|
|
|
Oct 15, 2009 |
|
Olena Onopriyenko |
UT |
Expression_protocol_Toronto |
success |
E. coli |
BL21-CodonPlus(DE3)-RIL |
Se-Met |
1 |
37 |
20 |
none |
production |
100 |
100 |
View Edit Delete |