| Id |
Target |
Expression Suffix |
Clone |
Local Clone |
Local Protein Target |
Local Expression |
Experiment Date Start |
Experiment Date End |
Staff |
Lab |
Protocol |
Status |
Expression Organism |
Expression Strain |
Media Type |
Media Volume |
Growth Temperature |
Induction Temperature |
Induction Reagent |
Experiment Type |
Expression Level |
Solubility Level |
Actions |
|
2768 |
IDP01108 |
2 |
2435 |
|
|
J0002338 |
Sep 16, 2008 |
Sep 17, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
3431 |
IDP01109 |
1 |
3098 |
|
|
J0002339 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
3391 |
IDP01140 |
1 |
3058 |
|
|
J0002401 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
292 |
IDP00415 |
2 |
291 |
|
|
J0000677 |
Jun 16, 2008 |
Jun 17, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9293 |
IDP04638 |
1 |
6521 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
0 |
View Edit Delete |
|
2988 |
IDP00961 |
1 |
2655 |
|
|
J0002059 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9306 |
IDP04651 |
1 |
6534 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
2693 |
IDP01155 |
2 |
2360 |
|
|
J0002434 |
Sep 16, 2008 |
Sep 17, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9308 |
IDP04653 |
1 |
6536 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
10 |
0 |
View Edit Delete |
|
1645 |
IDP00180 |
5 |
1758 |
|
|
J0001645 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
5766 |
IDP00901 |
3 |
2575 |
|
|
|
Oct 13, 2008 |
Oct 14, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
5767 |
IDP00901 |
4 |
1977 |
|
|
|
Oct 14, 2008 |
Oct 15, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
5880 |
IDP01009 |
3 |
2723 |
|
|
|
Oct 13, 2008 |
Oct 14, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
2537 |
IDP01087 |
2 |
2204 |
|
|
J0002312 |
Sep 16, 2008 |
Sep 17, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9175 |
IDP04459 |
1 |
6403 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
3299 |
IDP01156 |
1 |
2966 |
|
|
J0002435 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
6831 |
IDP90096 |
1 |
4534 |
|
|
|
Feb 12, 2009 |
Feb 13, 2009 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
0 |
View Edit Delete |
|
6826 |
IDP90091 |
1 |
4529 |
|
|
|
Feb 12, 2009 |
Feb 13, 2009 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
0 |
View Edit Delete |
|
5806 |
IDP00939 |
3 |
2623 |
|
|
|
Oct 13, 2008 |
Oct 14, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
2956 |
IDP00939 |
1 |
2623 |
|
|
J0002015 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
0 |
View Edit Delete |