Clone

Target

IDP00044  

Clone Suffix

2  

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

265  

Reference Sequence Used

MNDIVASTQL PNTIKTITND LRKLGLKKGM TVIVHSSLSS IGWISGGAVA VVEALMEVIT EEGTIIMPTQ SSDLSDPKHW SRPPVPEEWW QIIRDNVPAF EPHITPTRAM GKVVECFRTY PNVVRSNHPL GSFAAWGRHA EEITVNQSLS MSLGEESPLR KIYDLDGYIL LIGVGYDSNT SVHLSEVRSG ACELIKVGAP IIENGERVWK EFVDMDYDSD KFVEIGVEFE QKGTVTMGKI GNAKCRLMKQ RDIVDFGTEW FRKKN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MNDIVASTQL PNTIKTITND LRKLGLKKGM TVIVHSSLSS IGWISGGAVA VVEALMEVIT EEGTIIMPTQ SSDLSDPKHW SRPPVPEEWW QIIRDNVPAF EPHITPTRAM GKVVECFRTY PNVVRSNHPL GSFAAWGRHA EEITVNQSLS MSLGEESPLR KIYDLDGYIL LIGVGYDSNT SVHLSEVRSG ACELIKVGAP IIENGERVWK EFVDMDYDSD KFVEIGVEFE QKGTVTMGKI GNAKCRLMKQ RDIVDFGTEW FRKKN  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATGACATAGTGGCAAGTA  

PCR Backward Primer

TTATCCACTTCCAATGCTAATTTTTCTTACGAAACCATTC  

Shipped To Lab

UT  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008