Clone

Target

IDP00167  

Clone Suffix

2  

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

4558  

Reference Sequence Used

MVFYLIPKRR VWLMGKPFWR SVEYFFTGNY SADDGNNNIV AIGFGGQIHA YGGDDHVTVG SIGATVYTGS GNDTVVGGSA YLKVEDSTGH LIVKGAAGYA DINKSGDGNV SFAGAAGGVS IDHLGNHGDV SYGGAAAYNG ITRKGLSGNV TFAGAGGYNA LWHETNQGNL SFTGAGAGNK LDRTWSNRYQ GSHGDVTFDG AGAANSISSR VETGNITFRG AGADNHLVRK GKVGDITLQG AGASNRIERT HQAEDVYTQT RGNIRFEGVG GYNSLYSDVA HGDIHFSGGG AYNTIIRKGS GNDFAKEGMT NAKADEIVLT KAVMSGSWIG QDHHVTAVKS ASEPNTYLFA FADSTYTKIN KVQLRNDPQT GELKYYSTAW YKEVNHLSNL ANQDISDNGG FTAVNINGAY TLSDLKVEHQ QSVTVHAVEK SLTEYEWVTY ANGAVIDAKE VSLSDAKMGG HAIYADGTKV DVKAVKSNRQ PNTYIYAKVL GPYTKIVVVE LANDPETGAL KYQARSWYKE GDHTANIANQ DISSATGYNP MGKGGYSLSD LHYSVNAVRS TSETVADIEE YTDQTLFKPA NDSGESSGDV RFNGAGGGNV IKSNVTRGNV HFNGGGIANV ILHSSQFGNT EFNGGGAANV IVKSGEEGDL TFRGAGLANV LVHQSEQGKM DVYAGGAVNV LVRLGDGQYL AHLLAYGNIS VQKGSGDSRV VMLGGYNTHT QIGSGNGLWL AAGGFNVMTQ VGKGDVAAVL AGGANVLTKM GEGELTSGML GGANVITHIS NDDQLSNTTA VALGGANILT KKGKGNTLAV MGGGANVLTH VGDGTTTGVM VGGANILTKV GNGDTTGILL GVGNVLTHVG DGQTLGVMGA AGNIFTKVGD GTSIAVMIGA GNIFTHVGEG NAWALMGGLG NVFTKVGNGD ALALMVAEAN VFTHIGDGMS VALMLAKGNV ATKVGNGTTL AAMVGNVNIF THIGHGSTFA AMIGQANIMT KVGNDLTAAL MVGKANIMTH VGDGTSLGLF AGEVNVMTKV GNGTTLAAMF GKANIMTHVG DGLTGVLALG EANIVTKLGD DFMGVVAAAK ANVVTHVGDA TTAAVLAGKG NILTKVGEGT TVGLLISDVG NVMTHVGDGT TIGIAKGKAN LITKVGDGLG VNVTWGQANV FTQVGDGDRY NFAKGEANLI TKVGDGQEVS VVQGEANIIT HVGNGDDYTG AWGKANVITK VGHGQNVVLA KGEANIVTQV GDGDSFNALW SKGNIVTKVG DGMQVTAAKG QANITTTVGN GLNVTAAYGD ANINTKVGDG VSVNVAWGKY NINTKVGDGL NVAVMKGKAN ANIHVGDGLN INASYAQNNV AIKVGNGDFY SLAVASSNTS SNKLSALFDN IKQTVLGVGG SQAINYLVQG DEASSSGTHK GRGAIATPEI TKLDGFQMDA IKEVSSDLGD SLTGSVTKVD TPDLNKMQHA LNVDDSSVQA PNLIVNGDFE LGEHGWQSTH GVEASYAGSV YGVEGEGHGA RVTELDTYTN TSLYQDLANL AQGEVIAVSF DFAKRAGLSN NEGIEVLWNG EVVFSSSGDE SAWQQKNLKL TAQAGSNRIE FKGTGHNDGL GYILDNVVAT SESSQQANAI REHATQNPAA QNALSDKERA EADRQRLEQE KQKQLDAVAG SQSQLESTDQ QALENNGQAQ RDAVKEESEA VTAELAKLAQ GLDVLDGQAT HTGESGDQWR NDFAGGLLDG VQSQLDDAKQ LANDKIAAAK QTLSDNNSKV KESVAKSEAG VAQGEQNRAG VEQDIADAQA DAEKRKADAL AKGKDAQQAE SDAHHAVNNA QSRGDRDVQL AENKANQAQA DAQGAKQNEG DRPDRQGVTG SGLSGNAHSV EGAGETDSHV NTDSQTNADG RFSEGLTEQE QEALEGATNA VNRLQINAGI RAKNSVSSMT SMFSETNSKS IVVPTKVSPE PERQEVTRRD VRISGVNLES LSAVQGSQPT GQLASKSVPG FKSHFASTSI GIENELSGLV VVLPKNSAQT FGYVHDSQGN PLFMLTKDMN QGGYSNPVGI NDIQGVNNWQ THTIELVTYP SEISDTAAVE SRKEAMLWLA KEFTDHINQS NHQSLPHLVS DDGRFTLVIS NSKHLIAAGN GTSIDAQGKT IGMTPSGQQA TMAISAKEFG TSSSPEVRLL ESAPWYQAGL RDEFLANAKN TTLDDPATAQ NVYAYLTSVY SKTADLAKEY GIYINDWDPA SEGFSPNAQG LTDPKVKNAW SILPRTKPVR MLELLSAEDS RYVRQQIAEK LKGTYSESLA KNVFEYFQYG GEVAGHGINN ATTGSVQQPE PAILFEFRSV PSALSDFVPK TASTVKVDVK ALDHFDSASR KAIITEVNAL VSGSEDFDAW YQEYRASKGQ PPVKNPKSSA SANHKAEWLM TQHAEQWAKI TAPYTDNHET LTSTKLASND KEELHALGET SNLENNKQQE NVASIINTML NDMLPFYALR TERNLLVQEG DEGFEVRAWP GTEDKSKTII LEDPEDAAQH KAIERFILAN FDNFEQMPDE LFLVDNKVIS HHEGRTHVLA QKVDGAWQYN ATVELMSVTE LLDAANVTGK IRGESYQQVI DALTDYHASI TEHADYEPES VEKLLNLRKK IEGYVLGHPD SGRVEAMNSL LNQVNTRLDE VSLLSVAEQT IQAQNSFSRL YDQLEAANLK ESKHLYLDQN GDFVTKGKGN LANIDLLGSR EAVLEKVKLT VSNEYGQTVA DTIFAGLSAK DLAKDGKGVD IAGLNKVHQA IEQHLSPVSA TLYIWKPSDH SALGHAALQI GQGRTQLEGQ AAADFNQQNY VSWWPLGSKS SNISNILNVA TKDQPDLKLR WSDFSQPAHQ NDTLEHDVAS EENDGFGLHD GDIKLKRFIE KLNAAKGIDA SFKEASEGYA SVLLGNPDML ETTSIPAHVF QPFVEQWNDT SYDMMDVAHR FAQELRLQAQ RSDDPELLEK RIGNVIRQFA ERALEEIETF KASQADQGRV FRINLEGLDV AAMQAEWHRL SNDPDARYQL LTKNCSSTVA KVLKAGGADK LIGHTWLPKF GVWTPTELFN FGQALQEAQL EIAAKKQSHQ VTDVLDALSG NEKPKENVAI ENDGTPPRDK ESLSPLTRFL NNELYGDKEA RRKIGEITQT LLDHAVEKGE SQKITLQGEA GRLTGYYHQG TAPSEGETSS PSGKVVLFLH GSGSSAEEQA SAIRNHYQKQ GIDMLAVNLR GYGESDGGPS EKGLYQDART MFNYLVNDKG IDPSNIIIHG YSMGGPIAAD LARYAAQNGQ AVSGLLLDRP MPSMTKAITA HEVANPAGIV GAIAKAVNGQ FSVEKNLEGL PKETSILLLT DNEGLGNEGE KLRTKLTASG YNVTGEQTFY GHEASNRLMS QYADQIVSGL SSSASVDEDL DQQGLDTTST KDQGISNKND HLQVVDSKEA LADGKILHNQ NVNSWGPITV TPTTDGGETR FDGQIIVQME NDPVVAKAAA NLAGKHAESS VVVQLDSDGN YRVVYGDPSK LDGKLRWQLV GHGRDHSETN NTRLSGYSAD ELAVKLAKFQ QSFNQAENIN NKPDHISIVG CSLVSDDKQK GFGHQFINAM DANGLRVDVS VRSSELAVDE AGRKHTKDAN GDWVQKAENN KVSLSWDAQG EVVAKDERIR NGIAEGDIDL SRIGVNNVDE PARGAIGDNN DVFDAPEKRK PETEVIANSS SSNQFSYSGN IQVNVGEGEF TAVNWGTSNV GIKVGTGGFK SLAFGDNNVM VHIGDGESKH SVXIGGYQAL EGAQMFLGNR NVSFNFGHSN DLILMMDKSI PTPPLVNPFD GAARISGVLQ GIATSGEGED WLAAQEQQWT LSGAKKFVKD MSGLDQSSSV DYTTLVELDS QNERDSRGLK HDAEATLNKQ YNQWLSGNGN SGTSQLSRAD KLRQANEKLA FNFAVGGQGX DIQVTTGXWN FMFGDNIQSI LDTNLGSLFG LMTQQFTATG QAKTTFTYTP QDLPRQLKNK LLGQLAGVGA ETTLADIFGV DYTASGQIVS RNGQAVDGVA ILKEMLEVIG EFSGDQLQAF VDPAKLLDSL KAGIDMGADG IKSFAETHGL KEKAPEEEKD NSSVSVNGAN VNSAQGATVA DGNTETAETQ DRAFGFNSLN LPNLFATIFS QDKQKEMKSL VENLKQNLTA DLLNMKEKTF DFLRNSGHLQ GDGDINISLG NYNFNWGGDG KDLGAYLGDN NNFWGGRGDD VFYATGKSNI FTGGEGNDMG VLMGRENMMF GGDGNDTAVV AGRINHVFLG AGDDQSFVFG EGGEIDTGSG RDYVVTSGNF NRVDTGDDQD YSVTIGNNNQ VELGAGNDFA NIFGNYNRIN AGAGNDVVKL MGYHAVLNGG DGDDHLIATA ISKFSQFNGG EGRDLMVLGG YQNTFKGGTD VDSFVVSGDV IDNLVEDIRS EDNIVFNGID WQKLWFERSG YDLKLSILRD PSNDSDQSKF EHIGSVTFSD YFNGNRAQVV IGMSEKDLSG EREYTMLSDS AIDALVQAMS GFEPQAGDNG FIDSLESKSQ AAISMAWSDV VHKKGLMV  

Reference Sequence Variance

TOOLONG  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGTTTTCTATTTAATCCCTAAA  

PCR Backward Primer

TTATCCACTTCCAATGTTACACCATCAATCCTTTTTTGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008