Clone

Target

IDP01038  

Clone Suffix

1  

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

159  

Reference Sequence Used

MRIGHGFDVH AFGGEGPIII GGVRIPYEKG LLAHSDGDVA LHALTDALLG AAALGDIGKL FPDTDPAFKG ADSRELLREA WRRIQAKGYT LGNVDVTIIA QAPKMLPHIP QMRVFIAEDL GCHMDEVNVK ATTTEKLGFT GRGEGIACEA VALLMKAAK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MRIGHGFDVH AFGGEGPIII GGVRIPYEKG LLAHSDGDVA LHALTDALLG AAALGDIGKL FPDTDPAFKG ADSRELLREA WRRIQAKGYT LGNVDVTIIA QAPKMLPHIP QMRVFIAEDL GCHMDEVNVK ATTTEKLGFT GRGEGIACEA VALLMKAAK  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCGAATTGGACACGGTTTT  

PCR Backward Primer

TTATCCACTTCCAATGTCATTTCGCCGCCTTCATCA  

Shipped To Lab

ANL  

Shipped Date

Oct 09, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008