Clone

Target

IDP90225  

Clone Suffix

1  

Sequence Comments

 

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

168  

Reference Sequence Used

MVRYPLEPVL SIKKDRVDRA EKVVKEKRRL LELEQEKLRE RESERDKVKN HYMQKIRQLR EQLDDGTTSD AILKMKAYIK VVAIQLSEEE EKVNKQKENV LAASKELERA EVELTKRRKE EEKTRLHKEE WMKEALKEEA RQEEKEQDEM GQLLHQLHKQ KQRESGEN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MVRYPLEPVL SIKKDRVDRA EKVVKEKRRL LELEQEKLRE RESERDKVKN HYMQKIRQLR EQLDDGTTSD AILKMKAYIK VVAIQLSEEE EKVNKQKENV LAASKELERA EVELTKRRKE EEKTRLHKEE WMKEALKEEA RQEEKEQDEM GQLLHQLHKQ KQRESGEN  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGTTAGATATCCTTTAGAACC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTTCTCCCCAGATTCCC  

Shipped To Lab

UT  

Shipped Date

Apr 30, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 21, 2009