Clone

Target

IDP90224  

Clone Suffix

1  

Sequence Comments

 

Experiment Start Date

Jul 22, 2009  

Experiment End Date

Jul 24, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

239  

Reference Sequence Used

MPIKKPCLKL NLDSLNVVRS EIPQMLSANE RLKNNFNILY NQIRQYPAYY FKVASNVPTY SDICQSFSVM YQGFQIVNHS GDVFIHACRE NPQSKGDFVG DKFHISIARE QVPLAFQILS GLLFSEDSPI DKWKITDMNR VSQQSRVGIG AQFTLYVKSD QECSQYSALL LHKIRQFIMC LESNLLRSKI APGEYPASDV RPEDWKYVSY RNELRSDRDG SERQEQMLRE EPFYRLMIE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCCCATAAAAAAGCCCTGT  

PCR Backward Primer

TTATCCACTTCCAATGCTACTCTATCATCAAACGATAAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 10, 2009