Clone

Target

IDP90525  

Clone Suffix

1  

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

231  

Reference Sequence Used

MNDPKVIVAL DYDNLADALA FVDKIDPSTC RLKVGKEMFT LFGPDFVREL HKRGFSVFLD LKFHDIPNTC SKAVKAAAEL GVWMVNVHAS GGERMMAASR EILEPYGKER PLLIGVTVLT SMESADLQGI GILSAPQDHV LRLATLTKNA GLDGVVCSAQ EASLLKQHLG REFKLVTPGI RPAGSEQGDQ RRIMTPAQAI ASGSDYLVIG RPITQAAHPE VVLEEINSSL V  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MNDPKVIVAL DYDNLADALA FVDKIDPSTC RLKVGKEMFT LFGPDFVREL HKRGFSVFLD LKFHDIPNTC SKAVKAAAEL GVWMVNVHAS GGERMMAASR EILEPYGKER PLLIGVTVLT SMESADLQGI GILSAPQDHV LRLATLTKNA GLDGVVCSAQ EASLLKQHLG REFKLVTPGI RPAGSEQGDQ RRIMTPAQAI ASGSDYLVIG RPITQAAHPE VVLEEINSSL V  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAACGACCCAAAAGTGATTG  

PCR Backward Primer

TTATCCACTTCCAATGCTAAACTAAAGAGCTGTTTATCT  

Shipped To Lab

NU  

Shipped Date

Nov 03, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009