Clone

Target

IDP90549  

Clone Suffix

1  

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

159  

Reference Sequence Used

MITKAIYPGT FDPITNGHLD LVTRASAMFS HVILAIADSS SKKPMFTLDE RVALAKKVTA PLKNVEVLGF SELMAEFAKK HNANILVRGL RSVSDFEYEW QLANMNRHLM PKLESVFLIP SEKWSFISSS LVKEVARHGG DITPFLPKPV TKALLAKLA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MITKAIYPGT FDPITNGHLD LVTRASAMFS HVILAIADSS SKKPMFTLDE RVALAKKVTA PLKNVEVLGF SELMAEFAKK HNANILVRGL RSVSDFEYEW QLANMNRHLM PKLESVFLIP SEKWSFISSS LVKEVARHGG DITPFLPKPV TKALLAKLA  

PCR Forward Primer

TACTTCCAATCCAATGCGATGATCACCAAAGCCATCTATC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGGCCAATTTGGCCAATAAT  

Shipped To Lab

ANL  

Shipped Date

Nov 03, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009