Clone

Target

IDP90611  

Clone Suffix

1  

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

212  

Reference Sequence Used

MNAKFIVIEG LEGAGKSTAI QVVVETLQQN GIDHITRTRE PGGTLLAEKL RALVKEEHPG EELQDITELL LVYAARVQLV ENVIKPALAR GEWVVGDRHD MSSQAYQGGG RQIAPSTMQS LKQTALGDFK PDLTLYLDID PKLGLERARG RGELDRIEKM DISFFERARE RYLELANSDD SVVMIDAAQS IEQVTADIRR ALQDWLSQVN RV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MNAKFIVIEG LEGAGKSTAI QVVVETLQQN GIDHITRTRE PGGTLLAEKL RALVKEEHPG EELQDITELL LVYAARVQLV ENVIKPALAR GEWVVGDRHD MSSQAYQGGG RQIAPSTMQS LKQTALGDFK PDLTLYLDID PKLGLERARG RGELDRIEKM DISFFERARE RYLELANSDD SVVMIDAAQS IEQVTADIRR ALQDWLSQVN RV  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAACGCAAAATTTATCGTAATT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGACACGGTTAACTTGACTC  

Shipped To Lab

NU  

Shipped Date

Nov 03, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009