Clone

Target

IDP90711  

Clone Suffix

1  

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

298  

Reference Sequence Used

MDKNIIIGAM TALITPFKNG KVDEQSYARL IKRQIENGID AVVPVGTTGE SATLTHEEHR TCIEIAVETC KGTKVKVLAG AGSNATHEAV GLAKFAKEHG ADGILSVAPY YNKPTQQGLY EHYKAIAQSV DIPVLLYNVP GRTGCEISTD TIIKLFRDCE NIYGVKEASG NIDKCVDLLA HEPRMMLISG EDAINYPILS NGGKGVISVT SNLLPDMISA LTHFALDENY KEAKKINDEL YNINKILFCE SNPIPIKTAM YLAGLIESLE FRLPLCSPSK ENFAKIEEVM KKYKIKGF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MDKNIIIGAM TALITPFKNG KVDEQSYARL IKRQIENGID AVVPVGTTGE SATLTHEEHR TCIEIAVETC KGTKVKVLAG AGSNATHEAV GLAKFAKEHG ADGILSVAPY YNKPTQQGLY EHYKAIAQSV DIPVLLYNVP GRTGCEISTD TIIKLFRDCE NIYGVKEASG NIDKCVDLLA HEPRMMLISG EDAINYPILS NGGKGVISVT SNLLPDMISA LTHFALDENY KEAKKINDEL YNINKILFCE SNPIPIKTAM YLAGLIESLE FRLPLCSPSK ENFAKIEEVM KKYKIKGF  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGATAAAAATATTATCATTGGG  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAATCCTTTGATCTTATATTTTT  

Shipped To Lab

ANL  

Shipped Date

Nov 03, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009