Clone

Target

IDP90922  

Clone Suffix

1  

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

252  

Reference Sequence Used

MKTVTVRDLV VGEGAPKIIV SLMGKTITDV KSEALAYREA DFDILEWRVD HFANVTTAES VLEAAGAIRE IITDKPLLFT FRSAKEGGEQ ALTTGQYIDL NRAAVDSGLV DMIDLELFTG DDEVKATVGY AHQHNVAVIM SNHDFHKTPA AEEIVQRLRK MQELGADIPK IAVMPQTKAD VLTLLTATVE MQERYADRPI ITMSMSKTGV ISRLAGEVFG SAATFGAVKK ASAPGQISVA DLRTVLTILH QA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKTVTVRDLV VGEGAPKIIV SLMGKTITDV KSEALAYREA DFDILEWRVD HFANVTTAES VLEAAGAIRE IITDKPLLFT FRSAKEGGEQ ALTTGQYIDL NRAAVDSGLV DMIDLELFTG DDEVKATVGY AHQHNVAVIM SNHDFHKTPA AEEIVQRLRK MQELGADIPK IAVMPQTKAD VLTLLTATVE MQERYADRPI ITMSMSKTGV ISRLAGEVFG SAATFGAVKK ASAPGQISVA DLRTVLTILH QA  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAACCGTAACTGTAAGAG  

PCR Backward Primer

TTATCCACTTCCAATGTTACGCCTGGTGCAATATAGT  

Shipped To Lab

NU  

Shipped Date

Nov 03, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009