Target   IDP90258

Stage

in_pdb   : 3KY7

Locus Tag/Selection DB Lookup

SAR1216  

NCBI Protein Lookup

gi: 49483403   accession: YP_040627  

Organism

Staphylococcus aureus subsp. aureus MRSA252  

Common Name

putative tRNA (guanine-7-)-methyltransferase [Staphylococcus aureus subsp. aureus MRSA252]  

Gene Name

trmD  

NCBI Gene Lookup

2860396  

NCBI Annotation

Similar to Escherichia coli tRNA (guanine-7-)-methyltransferase TrmD SW:TRMD_ECOLI (P07020) (255 aa) fasta scores: E(): 7.7e-41, 44.398% id in 241 aa, and to Bacillus subtilis tRNA (guanine-7-)-methyltransferase TrmD SW:TRMD_BACSU (O31741) (243 aa) fasta scores: E(): 2.5e-63, 62.656% id in 241 aa  

TIGR Main Role/Annotations

 

TIGR Sub Role

 

Comment

(From requestor): we are in the process of developing chemical inhibitors. Currently have GST fusion protein construct. will be cloning into histidine tagged PET vector.  

Selection Phase

Community Requests  

Date Approved

Mar 20, 2009  

Justification for Selection

Potential drug target  

Justification Codes

 

Priority

8  

Sequence Length

245  

(Subtargets)

 

pI

5.57  

Molecular Weight

28 kDa  

Sequence

(Check Homologs)

MKIDYLTLFP EMFDGVLNHS IMKRAQENNK LQINTVNFRD YAINKHNQVD DYPYGGGQGM VLKPEPVFNA MEDLDVTEQA RVILMCPQGE PFSHQKAVEL SKADHIVFIC GHYEGYDERI RTHLVTDEIS MGDYVLTGGE LPAMTMTDAI VRLIPGVLGN EQSHQDDSFS DGLLEFPQYT RPREFKGLTV PDVLLSGNHA NIDAWRHEQK LIRTYNKRPD LIEKYPLTNA DKQILERYKI GLKKG  

DNA Sequence

 

Inserted

Mar 23, 2009