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Structure of IDP02733

Glucose-6-phosphate isomerase from Francisella tularensis.

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CSGID target
IDP02733 
PDB Id
3LJK (NCBI MMDB) 
Authors
J.Osipiuk,N.Maltseva,J.Hasseman,W.F.Anderson,A.Joachimiak,Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Jerzy Osipiuk 
Responsible lab
Argonne National Laboratory 
Deposition Date
Jan 26, 2010 
Release Date
Mar 16, 2010 

Annotation

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Description
Glucose-6-phosphate isomerase (also known as phosphoglucose isomerase (PGI)) is an enzyme that catalyzes the reversible aldose-ketose isomerization of glucose 6-phosphate (G6P) to fructose 6-phosphate (F6P). The reaction has direct roles in glycolysis and gluconeogenesis and, indirectly, other branches of carbohydrate metabolism such as the pentose phosphate pathway. PGI belongs to the aldose-ketose isomerase family of enzymes, all of which transfer a hydrogen atom between C1 and C2 of their respective substrates. PGIs use sugar substrates that exist in solution overwhelmingly in the hemiacetal or hemiketal ring form. PGIs also have a number of ‘moonlighting’ functions: it acts as an autocrine motility factor (AMF) and neuroleukin agent (NLK), a serine proteinase inhibitor and a differentiation and maturation mediator (DMM). It was found that GPI plays an essential role in the development of arthritis in mammals and may play roles in both cancer and autoimmunity. 
Functional assignment
Isomerase 
Slides:
  • Asymmetric unit
  • Biological unit
  • B-factors distribution
  • B-factors distribution on the surface
  • Homolog superposition
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    secondary structure, N to C, molecule

    Ligands

    Ligand code Name Ligand type
    IPA isopropyl alcohol
    MES 2-(n-morpholino)-ethanesulfonic acid
    GOL glycerol
    CA calcium ion
    MSE selenomethionine modified residue

    Structure information

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    Unit cell parameters

    Space Group
    P 32 2 1  
    Unit Cell

    a=115.06Å, b=115.06Å, c=84.45Å
    α=90.00, β=90.00, γ=120.00  
    Solvent content
    52.8  
    Matthews coefficient
    2.61  

    Refinement

    Data for the highest resolution shell is in parentheses.
    Resolution range
    32.30-1.48Å (1.52-1.48Å)  
    Rall(%)
    12.6  
    Rwork(%)
    12.4 (24.8)  
    Rfree(%)
    15.8 (30.4)  
    Num. observed reflections
    106466 (7227)  
    Num. Rfree reflections
    5323 (340)  
    Completeness(%)
    98.6 (91.3)  

    Model parameters

    Num Atoms
    5477  
    Num Waters
    722  
    Num Hetatoms
    0  
    Model mean isotropic B factor (Å2)
    11.580  
    RMSD bond length (Å)
    0.019  
    RMSD bond angle
    1.629°  
    Filename uploaded
    idp02733.pdb (uploaded on Jan 26, 2010 4:56 PM)  
    Inserted
    Jan 26, 2010