| Id |
Target |
Expression Suffix |
Clone |
Local Clone |
Local Protein Target |
Local Expression |
Experiment Date Start |
Experiment Date End |
Staff |
Lab |
Protocol |
Status |
Expression Organism |
Expression Strain |
Media Type |
Media Volume |
Growth Temperature |
Induction Temperature |
Induction Reagent |
Experiment Type |
Expression Level |
Solubility Level |
Actions |
|
9136 |
IDP04407 |
1 |
6364 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9137 |
IDP04410 |
1 |
6365 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
3391 |
IDP01140 |
1 |
3058 |
|
|
J0002401 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9135 |
IDP04406 |
1 |
6363 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
100 |
View Edit Delete |
|
9132 |
IDP04403 |
1 |
6360 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9133 |
IDP04404 |
1 |
6361 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
3390 |
IDP01229 |
1 |
3057 |
|
|
J0002581 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
7867 |
IDP02323 |
1 |
5252 |
|
|
|
Apr 29, 2009 |
Apr 30, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
10 |
100 |
View Edit Delete |
|
3299 |
IDP01156 |
1 |
2966 |
|
|
J0002435 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9129 |
IDP04396 |
1 |
6357 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
100 |
View Edit Delete |
|
7868 |
IDP02324 |
1 |
5253 |
|
|
|
Apr 29, 2009 |
Apr 30, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
100 |
View Edit Delete |
|
9063 |
IDP04287 |
1 |
6291 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
7378 |
IDP00137 |
1 |
4650 |
|
|
|
Apr 29, 2009 |
Apr 30, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
25 |
100 |
View Edit Delete |
|
9120 |
IDP04383 |
1 |
6348 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
100 |
View Edit Delete |
|
9121 |
IDP04384 |
1 |
6349 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
100 |
View Edit Delete |
|
9122 |
IDP04386 |
1 |
6350 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9123 |
IDP04387 |
1 |
6351 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
0 |
View Edit Delete |
|
9124 |
IDP04388 |
1 |
6352 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
100 |
View Edit Delete |
|
3352 |
IDP01176 |
1 |
3019 |
|
|
J0002475 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
10 |
0 |
View Edit Delete |
|
3433 |
IDP01174 |
1 |
3100 |
|
|
J0002471 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |