Center for Structural Genomics of Infectious Diseases

Expression

Target: IDP00038
Expression ID: A1075
Protein Clone: pMCSG7 (Apr 21, 2008)
Experiment Date Start: Jul 16, 2008
Experiment Date End: Jul 19, 2008
Person: Min Zhou
Lab: Argonne National Laboratory
Protocol: standard expression_ANL
Status: success
Expression Organism: E. coli
Expression Strain: BL21(DE3)magic
Media Type: M9 minimal + semet
Media Volume: 3000 mL
Growth Temperature (°C): 37
Induction Temperature (°C): 16
Induction Reagent: 1 mM IPTG
Experiment Type: production
Expression Level (%): 100
Solubility Level (%): 67
Inserted: Jul 29, 2011

Related Purifications

Experiment Start Date	Experiment End Date	Person	Lab	Protocol	Status	Affinity Tag Status	SeMet Status	Final Buffer	Shipped To Lab	Shipped Date
Aug 01, 2008	Aug 03, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-08-03
Aug 01, 2008	Aug 03, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-08-03
Aug 01, 2008	Aug 03, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-08-03
Aug 01, 2008	Aug 03, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-08-03
Aug 01, 2008	Aug 03, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-08-03
Aug 01, 2008	Aug 03, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-08-03
Aug 01, 2008	Aug 03, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-08-03