Expression
- Target
- IDP90533
- Expression ID
- J1
- Protein Clone
- pMCSG7 (Oct 28, 2009)
- Experiment Date Start
- Oct 28, 2009
- Experiment Date End
- Oct 28, 2009
- Person
- Sarah Grimshaw
- Lab
- J. Craig Venter Institute
- Protocol
- CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins
- Status
- success
- Expression Organism
- E. coli
- Expression Strain
- BL21(DE3)-Magic
- Media Type
- 2xYT
- Media Volume
- 0.15 mL
- Growth Temperature (°C)
- 37
- Induction Temperature (°C)
- 25
- Induction Reagent
- 1mM IPTG
- Experiment Type
- assay
- Expression Level (%)
- 30
- Solubility Level (%)
- 100
- Inserted
- Dec 03, 2009
