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Expression

Target
IDP04396  
Expression ID
J1  
Protein Clone
pMCSG7 (Nov 17, 2009)  
Local Clone ID
 
Local Protein Target ID
 
Local Expression ID
 
Experiment Date Start
Nov 30, 2009  
Experiment Date End
Dec 07, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)-Magic  
Media Type
2xYT  
Media Volume
0.15 mL  
Growth Temperature
37 °C  
Induction Temperature
25 °C  
Induction Reagent
1mM IPTG  
Experiment Type
assay  
Expression Level
30.0 %  
Solubility Level
100.0 %  
Inserted
Jan 08, 2010