Clone

Target

IDP00038  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

333  

Reference Sequence Used

MPLPKSLKYG DTIGIYSPSS PVTYTSPKRF ERAKSYLLQK GFHILEGSLT GRYDYYRSGS IQERAKELNA LIRNPNVSCI MSTIGGMNSN SLLPYIDYDA FQNNPKIMIG YSDATALLLG IYAKTGIPTF YGPALVPSFG EFEPFVDDTY KYFLETLLHD QALPYNIKQP LFWSDEFINW EEKTKEKELR PNNWISVTNG QATGRVIGGN LNTIQGIWGS PYMPCIQEGD ILFIEDSSKD AATIERSFSF LKINGVFDKV SGIILGKHEQ FDDCGTNRKP YEILLEVLQN QRIPLLADFD CCHTHPMITM PIGVQVKMDA TNKTIHILEK WKI  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MPLPKSLKYG DTIGIYSPSS PVTYTSPKRF ERAKSYLLQK GFHILEGSLT GRYDYYRSGS IQERAKELNA LIRNPNVSCI MSTIGGMNSN SLLPYIDYDA FQNNPKIMIG YSDATALLLG IYAKTGIPTF YGPALVPSFG EFEPFVDDTY KYFLETLLHD QALPYNIKQP LFWSDEFINW EEKTKEKELR PNNWISVTNG QATGRVIGGN LNTIQGIWGS PYMPCIQEGD ILFIEDSSKD AATIERSFSF LKINGVFDKV SGIILGKHEQ FDDCGTNRKP YEILLEVLQN QRIPLLADFD CCHTHPMITM PIGVQVKMDA TNKTIHILEK WKI  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCCACTTCCAAAATCATTAAA  

PCR Backward Primer

TTATCCACTTCCAATGTTATATCTTCCATTTCTCTAAAATA  

Shipped To Lab

ANL  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008