Clone

Target

IDP00203  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

257  

Reference Sequence Used

MNPLVIKLGG VLLDSEEALE RLFTALVTYR EKHERPLVIM HGGGCLVDEL MKRLALPVVK KNGLRVTPAD QIDIITGALA GTANKTLLAW AVKHQINAVG LCLADGNTVT VTLLDAELGH VGKAQPGSAA LVQTLLAAGY MPIISSIGIT VEGQLMNVNA DQAATALAAT LGADLILLSD VSGILDGKGQ RIAEMTAQKA EQLIAQGIIT DGMVVKVNAA LDAARSLGRP VDIASWRHSE QLPALFNGVP IGTRISV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAACCCGTTGGTCATTAAAT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGACCGAAATACGCGTACC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008