Clone

Target

IDP91447  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

180  

Reference Sequence Used

MAFVRKSGRK TQTTTEFEDL STPEHLIALA EDKGLETDPV NVSELAKHLG ITVRYEPMQD EESGSLKKEK KSGSWVMTVN SLHHPHRQRF TIAHEIAHRI RHAANNDCFE DTTFFRNGET NWMEAEANDF AASLLMPKDA FDNFIANVSS KVEDVAMHFQ VSSHAVRIRA KVLGYDGHNL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCATTCGTTAGAAAATCTG  

PCR Backward Primer

TTATCCACTTCCAATGTCATAGATTATGTCCGTCATAAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011