Clone

Target

IDP91571  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

169  

Reference Sequence Used

MDAFIVDPVQ GELYSGLSHT ELADIIRLAD SVENQLNGGN SFLDVFSTYM GQVISEFMHS NDNRIELLQR RLHSCSFLVN IEEMSYIDEA LQCPITLAIP QRGVFLRNAE GSRVCSLYDE MALSRIINDG MHHPLSREPI TLSMLVAREQ CEFDCSIGHF TVRSDCYSV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGACGCTTTTATTGTAGATC  

PCR Backward Primer

TTATCCACTTCCAATGCTACACTGAATAACAATCACTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011