Clone

Target

IDP91599  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

250  

Reference Sequence Used

MKVSVPGMPA TLLNMSNNDI YKMVSGDKMD MKMNIFQRLW ETLRHLFWSD KQTEAYKLLF NFVNKKAGNI NASKYFTGAV NENEKEKFIH SLELFNELKT CAKNPDEMVA KGNMSWVAQT FGDIELSVTF FIENKEICTQ TLQLHKGPGN LGVDLREAYL PGVDMRDCYL GLKTMKGHNK VLYLEPGWNA NLDGATLDGA TLDGATVDGA THLYDEVIII NKITPKKIDT EEVATKQSTA EQITDNAIIE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAGTATCAGTTCCAGGC  

PCR Backward Primer

TTATCCACTTCCAATGTCATTCAATAATTGCGTTGTCAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011