Clone

Target

IDP00330  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

416  

Reference Sequence Used

MFVKNVNFYY RQILEKFENS YFAEDLTKVI IGIDCDYLDA NELSFSEFKA KYYEALSKNK ICDFAGFFGV FSANFVSLFE KIPLSSKKNY DFPLFLFANA KAYLIYEKNS KMFFKFGASK YFEYLKDDIE PMKKKQKNDF EILNSLEDEK NDFLKMCEKA KEYLLSGDIF QVVLSKQLCI KHQVNAFDYY ESLSALNPSA YMFYFPSKYG VVLGSSPEFL LKIKKREIYL APIAGTRNLE NNCDLLALEK DLLSDEKELS EHKMLVDLAR NDASKFGTQT RVENLFSIIK NKFVMHIVSE VYANMKEDAS IFDVIEAVFP AGTLSGAPKI RALEITSELE DCDRGIYGGA VGFLNFNEDI TLAILIRCAF FTQDKAYLAS GAGIVLQSES QKEYAEICAK RKALLVAFEN LKKENQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTTGTTAAAAATGTAAATTTTTAT  

PCR Backward Primer

TTATCCACTTCCAATGTCATTGATTTTCCTTTTTTAAATTTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008