Clone

Target

IDP00348  

Clone Suffix

2 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

361  

Reference Sequence Used

MVNTNLEELR TQVDQLNIDL LELISKRANL VQEIGKIKGT QGSLRFDPLR EREMLNTILA ANEGPFEDST VQKLFKEIFK AGLELQEEDH SKALLVSRKN KKEDTIVTVK GLPIGNGEPV FVFGPCSVES YEQVAAVAES IKAKGLKLIR GGAFKPRTSP YDFQGLGLEG LKILKRVSDE YGLGVISEIV TPADIEVALD YVDVIQIGAR NMQNFELLKA AGRVDKPILL KRGLSATIEE FIGAAEYIMS QGNGKIILCE RGIRTYEKAT RNTLDISAVP ILKKETHLPV MVDVTHSTGR KDLLLPCAKA ALAIEADGVM AEVHPDPAVA LSDSAQQMDI PEFEEFWNAI LASNLVPHKI K  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MVNTNLEELR TQVDQLNIDL LELISKRANL VQEIGKIKGT QGSLRFDPLR EREMLNTILA ANEGPFEDST VQKLFKEIFK AGLELQEEDH SKALLVSRKN KKEDTIVTVK GLPIGNGEPV FVFGPCSVES YEQVAAVAES IKAKGLKLIR GGAFKPRTSP YDFQGLGLEG LKILKRVSDE YGLGVISEIV TPADIEVALD YVDVIQIGAR NMQNFELLKA AGRVDKPILL KRGLSATIEE FIGAAEYIMS QGNGKIILCE RGIRTYEKAT RNTLDISAVP ILKKETHLPV MVDVTHSTGR KDLLLPCAKA ALAIEADGVM AEVHPDPAVA LSDSAQQMDI PEFEEFWNAI LASNLVPHKI K  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGTTAATACAAATTTAGAGGAA  

PCR Backward Primer

TTATCCACTTCCAATGTTACTTTATTTTATGTGGTACTAAG  

Shipped To Lab

ANL  

Shipped Date

Oct 09, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008