Clone

Target

IDP00624  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

457  

Reference Sequence Used

MKIIKRAIIS LIILSLLISI TMSNASASEE LYYSVEYKNT ATFNKLVKKK SLNVVYNIPE LHVAQIKMTK MHANALANYK NDIKYINATC STCITSEKTI DRTSNESLFS RQWDMNKITN NGASYDDLPK HANTKIAIID TGVMKNHDDL KNNFSTDSKN LVPLNGFRGT EPEETGDVHD VNDRKGHGTM VSGQTSANGK LIGVAPNNKF TMYRVFGSKK TELLWVSKAI VQAANDGNQV INISVGSYII LDKNDHQTFR KDEKVEYDAL QKAINYAKKK KSIVVAAAGN DGIDVNDKQK LKLQREYQGN GEVKDVPASM DNVVTVGSTD QKSNLSEFSN FGMNYTDIAA PGGSFAYLNQ FGVDKWMNEG YMHKENILTT ANNGRYIYQA GTSLATPKVS GALALIIDKY HLEKHPDKAI ELLYQHGTSK NNKPFSRYGH GELDVYKALN VANQKAS  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAATCATAAAGCGCGCTA  

PCR Backward Primer

TTATCCACTTCCAATGTTAACTTGCTTTTTGATTTGCTAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008