Clone

Target

IDP01018  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

431  

Reference Sequence Used

MKTPRLLQYL AYPQKITGPI IEAELRDVAI GELCEIRRGW HQKQVVARAQ VVGLQRERTV LSLIGNAQGL SRDVVLYPTG RALSAWVGYS VLGAVLDPTG KIVERFTPEV APISEERVID VAPPSYASRV GVREPLITGV RAIDGLLTCG VGQRMGIFAS AGCGKTMLMH MLIEQTEADV FVIGLIGERG REVTEFVDML RASHKKEKCV LVFATSDFPS VDRCNAAQLA TTVAEYFRDQ GKRVVLFIDS MTRYARALRD VALASGERPA RRGYPASVFD NLPRLLERPG ATSEGSITAF YTVLLESEEE ADPMADEIRS ILDGHLYLSR KLAGQGHYPA IDVLKSVSRV FGQVTTPTHA EQASAVRKLM TRLEELQLFI DLGEYRPGEN IDNDRAMQMR DSLKAWLCQP VAQYSSFDDT LSGMNAFADQ N  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAACACCTCGTTTACTGC  

PCR Backward Primer

TTATCCACTTCCAATGTTAATTCTGGTCAGCGAATGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008