Clone

Target

IDP01062  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

337  

Reference Sequence Used

MSTHEATLQQ PLLRAIDLKK HYPVKKGIFS PERLVKALDG VSFNLERGKT LAVVGESGCG KSTLGRLLTM IETPTGGELY YQGQDLLKHD PHAQKLRRQK IQIVFQNPYG SLNPRKKVGQ ILEEPLLINT SLSKAQRREK ALAMMAKVGL KTEHYDRYPH MFSGGQRQRI AIARGLMLDP DVVIADEPVS ALDVSVRAQV LNLMMDLQQD MGLSYVFISH DLSVVEHIAD EVMVMYLGRC VEKGTKEQIF NNPRHPYTQA LLSATPRLNP DDRRERIKLT GELPSPLNPP PGCAFNARCS RRFGPCTQLQ PQLKEYDGQL VACFAVDQDE NPQKPLS  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGTACGCATGAGGCCAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAACTCAGCGGTTTCTGCG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008