Clone

Target

IDP90140  

Clone Suffix

1

Sequence Comments

Removed signal peptide from N-terminus (MRSLIIVLLFPSIIYS) and the endoplasmic reticulum retention signal (KTEL) from the C-terminus. Added 6HIS tag at C-terminus.  

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

221  

Reference Sequence Used

MRSLIIVLLF PSIIYSMSIR RCEKTEEETW GLKIGLCIIA KDFYPERTDC SVHRPTASGG LITEGNGFRV VIYDQCTEPH DFIITDTQQT RLGSSHTYIK FSNMNTGVPS SIPKCSRTLS ISVYCDQEAG DIKFEEYTQE SSDISIRVKY DSSCIDYLGI NQSFMNECIR RITTWDRESC VRIDTQTINK YLKSCTNTKF DRNVYKRYIL KSKALHAKTE L  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGGTCCCTGATTATAGTC  

PCR Backward Primer

TTATCCACTTCCAATGTTACAACTCTGTTTTAGCATGG  

Shipped To Lab

WU  

Shipped Date

Feb 02, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Feb 23, 2009