Clone

Target

IDP02331  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jan 28, 2009  

Experiment End Date

Jan 29, 2009  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

388  

Reference Sequence Used

MKTCSKITCA SLSLLIAGSS LLYITPASIV KAEPTQNVSS SLQTSNQSDR TSVKKAMRDS LQLGYPGILA QISKGGKNWS YAAGIADLRT KKQMKTDFRF RIGSTTKTFI ATVLLQLAGE NRLNLDDSIE KWLPGVIQGN GYDANQITIR QILNHTSGIA EYLKSKDYDI MDTKKLYTAE ELVKMGISLP PDFAPGKGWS YSNTGYVILG ILIEKVTGNS YAEEVENRIV EPLELSNTFL PGNSTVIPGT KHARGYERYD GESELKDVTY SYPGSSDGDM ISTADDLNKF FSYLLSGKLL KEQQLKQMLT TVPTGIAEIG RYGLGIYETK LPNGVSIWGH AGASPGFSTF AGGTLGGKHT VAINLNGHET SYSPSDPFKN ILLAEFSK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAACATGCAGTAAAATTACA  

PCR Backward Primer

TTATCCACTTCCAATGCTACTTGCTAAATTCAGCAAGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Feb 24, 2009