Clone

Target

IDP90546  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

279  

Reference Sequence Used

MKLCVALDLS TKEECLQLAK ELKNLDIWLK VGLRAYLRDG FKFIEELKKV DDFKIFLDLK FHDIPNTMAD ACEEVSKLGV DMINIHASAG KIAIQEVMTR LSKFSKRPLV LAVSALTSFD EENFFSIYRQ KIEEAVINFS KISYENGLDG MVCSVFESKK IKEHTSSNFL TLTPGIRPFG ETNDDQKRVA NLAMARENLS DYIVVGRPIY KNENPRAVCE KILNKIHRKN ISENDIEQNY EVIQQKEWDM CNHFEEWIKT RPDKEHALKE FYAKCGIKY  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKLCVALDLS TKEECLQLAK ELKNLDIWLK VGLRAYLRDG FKFIEELKKV DDFKIFLDLK FHDIPNTMAD ACEEVSKLGV DMINIHASAG KIAIQEVMTR LSKFSKRPLV LAVSALTSFD EENFFSIYRQ KIEEAVINFS KISYENGLDG MVCSVFESKK IKEHTSSNFL TLTPGIRPFG ETNDDQKRVA NLAMARENLS DYIVVGRPIY KNENPRAVCE KILNKIHRKN ISENDIEQNY EVIQQKEWDM CNHFEEWIKT RPDKEHALKE FYAKCGIKY  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAGCTTTGTGTCGCACTT  

PCR Backward Primer

TTATCCACTTCCAATGTCAATATTTTATCCCACACTTAG  

Shipped To Lab

NU  

Shipped Date

May 03, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009