Clone

Target

IDP00796  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

308  

Reference Sequence Used

MSKTAIIFPG QGAQKVGMAQ DLFNNNDQAT EILTSAANTL DFDILETMFT DEEGKLGETE NTQPALLTHS SALLAALKNL NPDFTMGHSL GEYSSLVAAD VLSFEDAVKI VRKRGQLMAQ AFPTGVGSMA AVLGLDFDKV DEICKSLSSD DKIIEPANIN CPGQIVVSGH KALIDELVEK GKSLGAKRVM PLAVSGPFHS SLMKVIEEDF SSYINQFEWR DAKFPVVQNV NAQGETDKEV IKSNMVKQLY SPVQFINSTE WLIDQGVDHF IEIGPGKVLS GLIKKINRDV KLTSIQTLED VKGWNEND  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MSKTAIIFPG QGAQKVGMAQ DLFNNNDQAT EILTSAANTL DFDILETMFT DEEGKLGETE NTQPALLTHS SALLAALKNL NPDFTMGHSL GEYSSLVAAD VLSFEDAVKI VRKRGQLMAQ AFPTGVGSMA AVLGLDFDKV DEICKSLSSD DKIIEPANIN CPGQIVVSGH KALIDELVEK GKSLGAKRVM PLAVSGPFHS SLMKVIEEDF SSYINQFEWR DAKFPVVQNV NAQGETDKEV IKSNMVKQLY SPVQFINSTE WLIDQGVDHF IEIGPGKVLS GLIKKINRDV KLTSIQTLED VKGWNEND  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGTAAAACAGCAATTATTTTTC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTCATTTTCATTCCATCCTT  

Shipped To Lab

NU  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008