Clone

Target

IDP00835  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

255  

Reference Sequence Used

MIDIHNHILV DIDDGPKTIE KSIALLKQAK DEGVTSIVAT PHHLHPRYDN TFQQVLVKLA ELRTHPEVQA LDIKLFPGQE IRITDSILKG LDNGSIQGIN RSKYLLIEFP TGEVPHYTKQ LFFEIQSRGY IPIIAHPERN RSIAKNPEIL YELVANGALS QLTSSSLVGG FGKNIQKLSL QFIECNLAHF VASDAHSCDQ RPFLMQELFH NHKLKKYSND IEALLRNASS VINDNFVYLD RPTKPGKVKS FLKWF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTGATATTCATAACCATATAT  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAACCATTTCAAAAACGACTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008