Clone

Target

IDP00340  

Clone Suffix

4 - The clone is available in BEIR and it is in stock.

Sequence Comments

477: L -> S  

Experiment Start Date

Apr 15, 2010  

Experiment End Date

Apr 15, 2010  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

612  

Reference Sequence Used

MCGIVGANST RNVTNILIEG LKKLEYRGYD SAGLAIIDDK NNIDICKEVG KVIELEKSVH NLANFKGDIG IAHTRWATHG KPSKNNSHPH ASESFCIVHN GVIENFAELK KVLINDGYKF KSDTDTEVIA HLLQKEWRDN FSIVDNIKYI MAMLKGAYAV AIISQKFSDK IVAVRSGSPL VIGVGIDENF ISSDALSLLP VTNKFSYLDE GDIAIISKDN VEVFDNNGAA KNLEVEEYNY SSSSASKDGY KHYMLKEIYE QPEAVSNTIL ASLADGEISL DSFDKRAKEL FEKTKHICIV ACGTSYNAGM TAKYWIEKYA KVPCSVEIAS EIRYRDNVVV DGSLFVSISQ SGETADTLES LRKSKKQNYV GSMCICNVPN SSLVRESDIA FMTKAGVEIG VASTKAFTTQ LVALAIFTLV IAKLKNSLTD QQIAKYTEEL KNIRALVMGA LKLDTEIDQI SEYFSDKEHT IFLGRGLYYP IAIEGALKLK EISYIHAEAY PSGELKHGPL ALVDKNMPIV AVVPNDELLD KTLSNLQEVH ARGGKLILFV DKAVKERVNF DNSIVLELDA GHDFSAPVVF TIPLQLLSYH VAIIKGTDVD QPRNLAKSVT VE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MCGIVGANST RNVTNILIEG LKKLEYRGYD SAGLAIIDDK NNIDICKEVG KVIELEKSVH NLANFKGDIG IAHTRWATHG KPSKNNSHPH ASESFCIVHN GVIENFAELK KVLINDGYKF KSDTDTEVIA HLLQKEWRDN FSIVDNIKYI MAMLKGAYAV AIISQKFSDK IVAVRSGSPL VIGVGIDENF ISSDALSLLP VTNKFSYLDE GDIAIISKDN VEVFDNNGAA KNLEVEEYNY SSSSASKDGY KHYMLKEIYE QPEAVSNTIL ASLADGEISL DSFDKRAKEL FEKTKHICIV ACGTSYNAGM TAKYWIEKYA KVPCSVEIAS EIRYRDNVVV DGSLFVSISQ SGETADTLES LRKSKKQNYV GSMCICNVPN SSLVRESDIA FMTKAGVEIG VASTKAFTTQ LVALAIFTLV IAKLKNSLTD QQIAKYTEEL KNIRALVMGA LKLDTEIDQI SEYFSDKEHT IFLGRGSYYP IAIEGALKLK EISYIHAEAY PSGELKHGPL ALVDKNMPIV AVVPNDELLD KTLSNLQEVH ARGGKLILFV DKAVKERVNF DNSIVLELDA GHDFSAPVVF TIPLQLLSYH VAIIKGTDVD QPRNLAKSVT VE  

PCR Forward Primer

TACTTCCAATCCAATGCGATGTGTGGAATAGTAGGTGCT  

PCR Backward Primer

TTATCCACTTCCAATGTTACTCAACGGTTACAGATTTAG  

Shipped To Lab

ANL  

Shipped Date

Apr 29, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010