Protocol

Short Name

CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins  

Full Name

JCVI_OverExpression_HT_protocol  

Type

expression  

Lab

J. Craig Venter Institute  

Description

Transformation, Frozen Culture and Overexpression of Recombinant Proteins as described by the SOP CSG-004 (see CSG-004_Trans, FC and OE_102708_v001-1.pdf). Lysis of Expression Cells by Sonication ; Preparation for Caliper as described by the SOP CSG-005 (see CSG-005_Lysis_by_Sonication_and_Caliper_Prep_102708_v001.pdf ).