Center for Structural Genomics of Infectious Diseases

Purifications

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Page 1 of 338, showing 20 records out of 6752 total, starting on record 1, ending on 20

Id	Target	Purification Suffix	Expression	Local Expression	Local Protein Target	Local Purification	Experiment Date Start	Experiment Date End	Staff	Lab	Protocol	Status	Description	Affinity Tag Status	Semet Status	Final Buffer	Final Concentration	Final Volume	Shipped To Lab	Shipped Date	Actions
133	IDP00258	1	2123	W1	Montana Myotis Leukoencephalitis Envelope Protein	W1	Sep 15, 2008	Sep 17, 2008	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.	uncleaved	semet	25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide	7.5	200	WU	2008-09-17	View Edit Delete
135	IDP00260	1	7385	W1	OMCP Monkeypox (N3R)	W1	Aug 25, 2008	Aug 26, 2008	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column. 4 hours denaturation at room temp. Works in co-refolding NKG2D.	uncleaved	semet	25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide	14	300			View Edit Delete
1019	IDP90123	1	6853	W1	MPXV_ZAI1979_005_035	W1	Feb 26, 2009	Feb 28, 2009	Chris Nelson	WU	WU_native_soluble_expression_1	success	6HIS purification on profinia, followed by gel filtration on Superdex 200.	cleaved	semet	25 mM HEPES ph=7.5, 150 mM NaCl	21.8	500	WU	2009-02-28	View Edit Delete
1545	IDP90521	1	8397				Nov 17, 2009		Charitha Nakka	UTSW	UTSW_standard purification	success	Extended the dialysis step by adding the additonal TEV Protease to the dialysis bag for 3hrs.	cleaved	native	dialysis buffer II - 500mM NaCl +50mM HEPES+1mM DTT	71.18	1500			View Edit Delete
1000	IDP00947	1	2964				Oct 20, 2008	Dec 02, 2008	Charitha Nakka	UTSW	UTSW_standard purification	success		cleaved	na	200mM NaCl, 100mM Hepes, 5% glycerol	8.3	3800			View Edit Delete
134	IDP00259	1	7384	W1	OMCP Protein Cowpox Virus	W1	Dec 15, 2007	Dec 16, 2007	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.	uncleaved	native	25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide	10.5	400			View Edit Delete
395	IDP00085	1	3520				Apr 04, 2008		Olena Onopriyenko	UT	Purification_protocol_Toronto	success		na	native		1.4	20			View Edit Delete
1018	IDP90117	1	6850	W1	MPXV_ZAI1979_005_027	W1	Feb 26, 2009	Feb 28, 2009	Chris Nelson	WU	WU_native_soluble_expression_1	success	6HIS purification on profinia, followed by gel filtration on Superdex 200.	cleaved	semet	25 mM HEPES ph=7.5, 150 mM NaCl	27.7	1000	WU	2009-02-28	View Edit Delete
2073	IDP05773	1	10979	W1	EVM166 rotein	W1	Feb 03, 2009	Feb 04, 2009	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.	uncleaved	native	20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide	15	100	WU	2009-02-04	View Edit Delete
379	IDP00253	1	2129	W1	Yellow Fever Virus Envelope Protein	W1	Oct 09, 2008	Oct 09, 2008	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column. Clean up over MonoQ HR5/5 in 20 mM Tris-HCL pH 8.0 using a 1M NaCl gradient.	uncleaved	semet	20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide	14	100	WU	2008-10-09	View Edit Delete
380	IDP00254	1	2130	W1	Japanese Encephalitis Virus Envelope Protein	W1	Oct 13, 2008	Oct 15, 2008	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column. Clean up over MonoQ HR5/5 in 20 mM Tris-HCL pH 8.0 using a 1M NaCl gradient.	uncleaved	semet	20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide	10	50	WU	2008-10-15	View Edit Delete
392	IDP00132	1	3521				Apr 04, 2008		Olena Onopriyenko	UT	Purification_protocol_Toronto	success		na	native		4.3	20			View Edit Delete
1020	IDP90121	1	4713	W2	MPXV_ZAI1979_005_031	W1	Mar 13, 2009	Mar 15, 2009	Chris Nelson	WU	WU_native_soluble_expression_1	success	6HIS purification on profinia, followed by gel filtration on Superdex 200.	cleaved	semet	25 mM HEPES ph=7.5, 150 mM NaCl	15	4780	WU	2009-03-15	View Edit Delete
1447	IDP02668	1	6724				Mar 30, 2009		Charitha Nakka	UTSW	UTSW_standard purification	success	We observed 2 peaks after Q-column. Pooled those fractions and run gel filtration independently. Observed 2 peaks for each gelfiltration column. Finally we ended up with 4 peaks. Pooled and concentrated all 4 peak fractions. Observed ceryals in B peak.	cleaved	na	Gel filtration Buffer (1L) - 200mM NaCl + 50mM Hepes + 1mM DTT	16.63	900			View Edit Delete
136	IDP00272	1	7395	W1	Dengue-1 Envelope Protein	W1	Feb 25, 2008	Feb 26, 2008	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column. 4 hours denaturation at room temp.	uncleaved	semet	25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide	12	200			View Edit Delete
409	IDP00257	1	3808	W1	Modoc Virus Envelope Protein	W1	Oct 31, 2008	Oct 31, 2008	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.	uncleaved	semet	20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide	15	150	WU	2008-10-31	View Edit Delete
1446	IDP02119	1	7282				Sep 28, 2009		Charitha Nakka	UTSW	UTSW_standard purification	success		cleaved	na	Dialysis buffer - 500mM NaCl, 50mM HEPES pH 7.5, 2.5% glycerol	34.66	500	UTSW		View Edit Delete
393	IDP00133	1	3522				Apr 04, 2008		Olena Onopriyenko	UT	Purification_protocol_Toronto	success		na	native		1.4	20			View Edit Delete
378	IDP00252	1	2121	W1	St. Louis Encephalitis Virus Envelope Protein	W1	Oct 08, 2008	Oct 14, 2008	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column. Clean up over MonoQ HR5/5 in 20 mM Tris-HCL pH 8.0 using a 1M NaCl gradient.	uncleaved	semet	20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide	12	100	WU	2008-10-14	View Edit Delete
381	IDP00280	1	2128	W1	Dengue-4 Envelope Protein	W1	Jun 08, 2008	Jun 10, 2008	Chris Nelson	WU	WU_native_inclusion_body_expression_2	success	Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.	uncleaved	native	25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide	10	50			View Edit Delete

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