Center for Structural Genomics of Infectious Diseases - Purifications

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Purifications

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Csgid ID: IDP00030

Page 1 of 1, showing 3 records out of 3 total, starting on record 1, ending on 3

Id	Target	Purification Suffix	Expression	Local Expression	Local Protein Target	Local Purification	Experiment Date Start	Experiment Date End	Staff	Lab	Protocol	Status	Description	Affinity Tag Status	Semet Status	Covalent Modification	Final Buffer	Final Concentration	Final Volume	Shipped To Lab	Shipped Date	Actions
5866	IDP00030	4873	12890	1073	WAp3/AkYQhMQpIDVt3cp1l4uzdQ	4873	Jul 21, 2008	Jul 23, 2008	Rory Mulligan	ANL	standard purification_ANL	success	IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	cleaved	semet		20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	14	1200	ANL	2008-07-23	View Edit Delete
8204	IDP00030	4872	12890	1073	WAp3/AkYQhMQpIDVt3cp1l4uzdQ	4872	Jul 21, 2008	Jul 23, 2008	Rory Mulligan	ANL	standard purification_ANL	success	IMAC-I, IMAC-II::ÃKTAxpress using IMAC-II program::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)	cleaved	semet		20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	0	25000	ANL	2008-07-23	View Edit Delete
8205	IDP00030	4871	12890	1073	WAp3/AkYQhMQpIDVt3cp1l4uzdQ	4871	Jul 21, 2008	Jul 23, 2008	Rory Mulligan	ANL	standard purification_ANL	success	IMAC-I, IMAC-II::ÃKTAxpress using IMAC-I program::ÃKTAxpress IMAC-I program eluate (250 mM imidazole)	cleaved	semet		20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	0	25000	ANL	2008-07-23	View Edit Delete

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