Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP00252
Purification ID: W4
Expression: AthenaES_SeMET (Sep 21, 2008)
Experiment Date Start: Jan 28, 2009
Experiment Date End: Jan 28, 2009
Person: Chris Nelson
Lab: Washington University
Protocol: WU_native_inclusion_body_expression_2
Status: success
Description: Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column. Clean up over MonoQ HR5/5 in 20 mM Tris-HCL pH 8.0 using a 1M NaCl gradient.
Affinity Tag Status: uncleaved
SeMet Status: semet
Covalent Modification
Final Buffer: 20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide
Final Concentration (mg/mL): 15
Final Volume (μL): 100
Shipped To Lab Id: WU
Shipped Date: Jan 28, 2008
Inserted: Mar 18, 2009

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	Screen	Screen Component	pH	Plate Type
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 3 % PEG 8000, 0.5 % Ethylene Glycol	add new	7		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 3 % PEG 8000, 1.0 % Ethylene Glycol	add new	8		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 2 % PEG 8000, 0.5 % Ethylene Glycol	add new	1		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 2 % PEG 8000, 1.0 % Ethylene Glycol	add new	2		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 2 % PEG 8000, 1.5 % Ethylene Glycol	add new	3		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 2 % PEG 8000, 2.0 % Ethylene Glycol	add new	4		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 2 % PEG 8000, 2.5 % Ethylene Glycol	add new	5		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 2 % PEG 8000, 3.0 % Ethylene Glycol	add new	6		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 3 % PEG 8000, 1.5 % Ethylene Glycol	add new	9		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 3 % PEG 8000, 2.5 % Ethylene Glycol	add new	11		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 3 % PEG 8000, 3.0 % Ethylene Glycol	add new	12		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 4 % PEG 8000, 0.5 % Ethylene Glycol	add new	13		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 4 % PEG 8000, 1.0 % Ethylene Glycol	add new	14		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 4 % PEG 8000, 1.5 % Ethylene Glycol	add new	15		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 4 % PEG 8000, 2.0 % Ethylene Glycol	add new	16		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 4 % PEG 8000, 2.5 % Ethylene Glycol	add new	17		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 4 % PEG 8000, 3.0 % Ethylene Glycol	add new	18		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 5 % PEG 8000, 0.5 % Ethylene Glycol	add new	19		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 5 % PEG 8000, 1.0 % Ethylene Glycol	add new	20		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 5 % PEG 8000, 1.5 % Ethylene Glycol	add new	21		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 5 % PEG 8000, 2.0 % Ethylene Glycol	add new	22		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 5 % PEG 8000, 2.5 % Ethylene Glycol	add new	23		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Na Acetate, 5 % PEG 8000, 3.0 % Ethylene Glycol	add new	24		Hampton_24_well
Feb 02, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Na Acetate, 3 % PEG 8000, 2.0 % Ethylene Glycol	add new	10	5.5	Hampton_24_well
Feb 28, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1M sodium acetate, 3%PEG 8000, 2% Ethylene Glycol	QIAGEN/PEGs