Purification

Target

IDP00254  

Purification ID

W1  

Expression

AthenaES_SeMET (Oct 03, 2008)  

Experiment Date Start

Oct 13, 2008  

Experiment Date End

Oct 15, 2008  

Person

Chris Nelson  

Lab

Washington University  

Protocol

WU_native_inclusion_body_expression_2  

Status

success  

Description

Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column. Clean up over MonoQ HR5/5 in 20 mM Tris-HCL pH 8.0 using a 1M NaCl gradient.  

Affinity Tag Status

uncleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide  

Final Concentration (mg/mL)

10  

Final Volume (μL)

50  

Shipped To Lab Id

WU  

Shipped Date

Oct 15, 2008  

Inserted

Oct 20, 2008