Purification
- Target
-
IDP00499
- Purification ID
-
A4943
- Expression
-
M9 minimal (Oct 15, 2008)
- Experiment Date Start
-
Jan 22, 2009
- Experiment Date End
-
Jan 24, 2009
- Person
-
Rory Mulligan
- Lab
-
Argonne National Laboratory
- Protocol
-
standard purification_ANL
- Status
-
success
- Description
-
IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
- Affinity Tag Status
-
cleaved
- SeMet Status
-
native
- Covalent Modification
-
- Final Buffer
-
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
- Final Concentration (mg/mL)
-
37
- Final Volume (μL)
-
1200
- Shipped To Lab Id
-
ANL
- Shipped Date
-
Jan 24, 2009
- Inserted
-
Jul 29, 2011
