Purification
- Target
-
IDP00038
- Purification ID
-
A6440
- Expression
-
M9 minimal + semet (Feb 28, 2011)
- Experiment Date Start
-
Feb 28, 2011
- Experiment Date End
-
Mar 02, 2011
- Person
-
Min Zhou
- Lab
-
Argonne National Laboratory
- Protocol
-
standard purification_ANL
- Status
-
success
- Description
-
IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
- Affinity Tag Status
-
na
- SeMet Status
-
semet
- Covalent Modification
-
- Final Buffer
-
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
- Final Concentration (mg/mL)
-
77
- Final Volume (μL)
-
600
- Shipped To Lab Id
-
ANL
- Shipped Date
-
Mar 02, 2011
- Inserted
-
Jul 29, 2011
