Purification
- Target
-
IDP90800
- Purification ID
-
A3450
- Expression
-
M9 minimal + semet (Nov 10, 2009)
- Experiment Date Start
-
Nov 18, 2009
- Experiment Date End
-
Nov 20, 2009
- Person
-
Min Zhou
- Lab
-
Argonne National Laboratory
- Protocol
-
standard purification_ANL
- Status
-
success
- Description
-
IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)
- Affinity Tag Status
-
cleavage unsuccessful
- SeMet Status
-
semet
- Covalent Modification
-
- Final Buffer
-
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
- Final Concentration (mg/mL)
-
206
- Final Volume (μL)
-
600
- Shipped To Lab Id
-
ANL
- Shipped Date
-
Nov 20, 2009
- Inserted
-
Jul 29, 2011
