Purification
- Target
-
IDP00038
- Purification ID
-
A4152
- Expression
-
M9 minimal + semet (Jul 16, 2008)
- Experiment Date Start
-
Aug 01, 2008
- Experiment Date End
-
Aug 03, 2008
- Person
-
Min Zhou
- Lab
-
Argonne National Laboratory
- Protocol
-
standard purification_ANL
- Status
-
success
- Description
-
IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-II program::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)
- Affinity Tag Status
-
cleaved
- SeMet Status
-
semet
- Covalent Modification
-
- Final Buffer
-
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
- Final Concentration (mg/mL)
-
2
- Final Volume (μL)
-
13000
- Shipped To Lab Id
-
ANL
- Shipped Date
-
Aug 03, 2008
- Inserted
-
Feb 27, 2012
