Target   IDP90286

Stage

in_pdb   : 3UKS

Locus Tag/Selection DB Lookup

TGME49_035700  

NCBI Protein Lookup

gi: 211966629   accession: EEB01825  

Organism

Toxoplasma gondii ME49  

Common Name

sedoheptulose-1,7 bisphosphatase, putative [Toxoplasma gondii ME49]  

Gene Name

 

NCBI Gene Lookup

 

NCBI Annotation

encoded by transcript TGME49_035700A; Contains Pfam PF00316: fructose-1,6-bisphosphatase Contains similarity to SwissProt:O20252 Sedoheptulose-1,7-bisphosphatase, chloroplast precursor (Sedoheptulose-bisphosphatase) (SBPASE) (SED(1,7)P2ASE), Spinacia oleracea; GO_function: GO:0042578 - phosphoric ester hydrolase activity; GO_process: GO:0005975 - carbohydrate metabolic process  

TIGR Main Role/Annotations

 

TIGR Sub Role

 

Comment

 

Selection Phase

Community Requests  

Date Approved

Apr 21, 2009  

Justification for Selection

Unpublished data indicate that the Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (TgSBPase) is plant plastid-like, but it is localized to the cytosol and not the 4-members parasite apicoplast. TgSBPase is essential for parasite survival by knock out studies, hence can serve as a parasite specific drug target.  

Justification Codes

 

Priority

8  

Sequence Length

330  

(Subtargets)

 

pI

5.23  

Molecular Weight

35.8 kDa  

Sequence

(Check Homologs)

MESPSALPSL DQLLKEQGAD QTLTDLILAI LDRCGKIASA LQGTSVDKVG SVNEFGDEQL TVDVIAENLL RSWAQSSEGS AVRAVCSEED IHLQECHKNG EFILCWDPLD GSSIIDCNWA VGSIVSIWRI GHHGVQWQGA DTLIQKTGRQ QVASLIVVYG PRTTGVVAVN VDAGGIVKEG TALDLEMKDN GKFICRGKPI IKPQAKIFSP ANLRAAQDLP AYKQLIEFWM EKRYTLRYTG GLVPDVYQIF VKQQGVFCNP ASKAAPAKLR MCFEVLAIAL VVEAAGGRTS NGQKSLLDVA IEHMDHRSAL CCGSADEIKR MEETFAALSG  

DNA Sequence

ATGGAG TCGCCT TCTGCA CTGCCA TCTCTG GATCAG CTTCTG AAGGAA CAAGGA GCAGAC CAGACA CTTACA GACCTC ATCTTG GCTATT CTTGAC AGATGC GGCAAA ATAGCG TCTGCG TTGCAA GGGACG TCAGTG GACAAA GTGGGC AGCGTC AATGAA TTCGGC GACGAA CAGCTG ACTGTC GACGTG ATCGCA GAGAAT CTTCTT CGGTCG TGGGCT CAGAGC AGCGAA GGCTCT GCGGTC CGCGCC GTCTGC AGTGAG GAGGAC ATCCAC CTTCAG GAATGC CATAAA AATGGA GAGTTT ATTTTG TGCTGG GACCCG CTCGAC GGAAGC AGCATC ATCGAC TGCAAC TGGGCT GTTGGA AGCATC GTGAGC ATCTGG CGAATC GGTCAC CATGGA GTCCAG TGGCAA GGCGCA GACACC CTGATT CAGAAA ACAGGG AGACAG CAGGTG GCGAGC TTGATT GTGGTG TACGGA CCTCGC ACGACG GGAGTC GTGGCG GTGAAC GTGGAC GCAGGC GGCATC GTCAAG GAGGGG ACCGCC CTCGAT TTGGAG ATGAAG GACAAC GGGAAG TTCATC TGCAGA GGCAAA CCGATC ATCAAG CCGCAG GCAAAG ATTTTC TCTCCA GCGAAT CTCCGT GCCGCG CAGGAC CTCCCG GCCTAC AAGCAG CTCATC GAGTTT TGGATG GAGAAG CGATAC ACGTTG AGATAC ACAGGA GGCCTC GTGCCG GACGTT TACCAG ATTTTC GTGAAG CAGCAA GGCGTC TTCTGC AATCCC GCGAGC AAGGCT GCTCCC GCCAAA CTGCGC ATGTGC TTTGAA GTCTTG GCAATC GCTCTC GTGGTC GAAGCG GCGGGC GGCCGA ACGAGC AACGGC CAGAAA TCTCTT CTCGAC GTGGCC ATTGAA CACATG GACCAT AGATCT GCTCTG TGCTGC GGAAGC GCTGAC GAAATC AAAAGA ATGGAG GAAACG TTTGCT GCACTC TCCGGC TAG  

Inserted

Jun 03, 2009