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Structure of IDP90722

Crystal Structure of Biotin Carboxylase-beta-gamma-ATP Complex fromCampylobacter jejuni

Edit deposit information
CSGID target
IDP90722 
PDB Id
3OUU (NCBI MMDB
Authors
N.Maltseva,Y.Kim,M.Makowska-Grzyska,R.Mulligan,L.Papazisi,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Natalia Maltseva 
Responsible lab
Argonne National Laboratory 
Deposition Date
Sep 15, 2010 
Release Date
Oct 20, 2010 

Annotation

Description
Biotin carboxylase, encoded by the accC gene, catalyzes the first step in fatty acid biosynthesis. It catalyzes the ATP-dependent transfer of carboxyl group from bicarbonate to biotin molecule of biotin carboxylase carrier protein (BCCP) . During second part of reaction transcarboxylase transfers the carboxyl group from carboxybiotinylated BCCP to acetyl-CoA to form malonyl-CoA and regenerate biotinylated BCCP. The formation of malonyl-CoA in turn controls the rate of fatty acid metabolism. Crystal structure of biotin carboxylase-beta-gamma-ATP complex from Campylobacter jejuni was solved at 2.1 A resolution. The structure is of an alpha-beta fold and protein molecules form dimer. Based on the structural peculiarities, the part of small domain (residues 133 - 202) swings back while interacting with adenosyl ligand to make compact patch. The binding region of biotin carboxylase is much conserved in different bacteria species. In accordance with what was found earlier in adenine-binding region Glu201-Leu204 and Ile157-Lys159 Leu204 is substituted by Ile204, ribose-binding region is formed by His209, Gln233, His236 and Ile437 is changed to Thr437, and His438 to Asn438. Phosphate groups of the ligand interact with glycine-rich group (residues 162-168) together with Lys116, Lys159 and Gln237 which interacts with the third phosphate group of the ligand.  
Functional assignment
 

Ligands

Ligand code Name Ligand type
CA calcium ion crystallization
CAC crystallization
AXP 4-acetamido-2,4-didexoy-d-glycero-beta-d-galacto-octopy ranosylphosphonic acid (an axial phosphonate) biological
GOL glycerol
FMT formate
MSE modified residue

Structure information

Unit cell parameters

Space Group
P 21 21 21  
Unit Cell

a=89.83Å, b=100.17Å, c=148.70Å
α=90.00, β=90.00, γ=90.00 
Solvent content
63.03  
Matthews coefficient
3.33  

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
44.92-2.25Å (2.33-2.25Å)  
Rall(%)
17.0 
Rwork(%)
16.8 (2350.0) 
Rfree(%)
22.0 (2890.0) 
Num. observed reflections
67052 (6247) 
Num. Rfree reflections
3399 (296) 
Completeness(%)
99.4 (99.0) 

Model parameters

Num Atoms
7784  
Num Waters
633  
Num Hetatoms
79  
Model mean isotropic B factor
28.890Å2  
RMSD bond length
0.011Å  
RMSD bond angle
1.309°  
RMSD dihedral angle
17.182°
 
Filename uploaded
dep.pdb (uploaded on Sep 17, 2010 8:47 AM)  
Inserted
Sep 17, 2010