Annotation

Description

Staphylococcus aureus is a ubiquitous pathogen causing infections in humans and domestic animals. It is often associated with bovine mastitis. Among secreted virulence factors, the leukotoxins constitute a family of toxins, which induce Ca2+ influx and pore formation that allows ethidium entry. SAOUHSC_00393 is a superantigen-like protein, type I toxin, which binds to a receptor on the cell surface and stimulates intracellular signaling pathways. These toxins are excreted by microoragism and cause damage to the host by destroying cells or disrupting normal cellular metabolism. Most exotoxins can be destroyed by heating and they are susceptible to antibodies produced by the immune system, but many exotoxins are so toxic that they may be fatal to the host before the immune system has a chance to mount defenses against it. 

Functional assignment

superantigen-like protein 

Structure information

Unit cell parameters

Space Group

P 21 21 21  

Unit Cell

a=51.70Å, b=72.31Å, c=123.96Å
α=90.00, β=90.00, γ=90.00 

Solvent content

 

Matthews coefficient

 

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

29.63-2.21Å (2.26-2.21Å)  

R_all(%)

20.8 

R_work(%)

20.5 (24.9) 

R_free(%)

25.2 (27.8) 

Num. observed reflections

23975 (1666) 

Num. R_free reflections

1222 (81) 

Completeness(%)

99.6 (95.0) 

Model parameters

Num Atoms

3363  

Num Waters

144  

Num Hetatoms

156  

Model mean isotropic B factor

52.600Ų  

RMSD bond length

0.012Å  

RMSD bond angle

1.569°  

Filename uploaded

rcsb064417.pdb (uploaded on Mar 24, 2011 12:14 PM)  

Inserted

Mar 24, 2011