Annotation

Description

The antibiotic vancomycin exerts its action by binding to an intermediate component of the bacterial cell wall synthesis pathway and inhibits the maturation of the cell wall. Vancomycin resistance type VanG is mediated by enzymes that modify peptidoglycan cell wall precursors such that they contain serine substituted for alanine; this single point mutation results in drastic decrease in the binding of vancomycin. One of the genes in the VanG operon is VanUg, which is a predicted transcription regulator of the expression of the VanG-type vancomycin resistance enzymes. This crystal structure shows VanUg adopts a helix-turn-helix (HTH) fold, typical of many bacterial transcription factors. This structure may assist with the understanding of the biology of VanG-type vancomycin resistance, and therefore may assist with the development of novel therapeutics to alleviate this type of antibiotic resistance. 

Functional assignment

transcription regulator 

Structure information

Unit cell parameters

Space Group

P 2 21 21  

Unit Cell

a=28.50Å, b=39.31Å, c=61.94Å
α=90.00, β=90.00, γ=90.00 

Solvent content

 

Matthews coefficient

 

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

33.19-1.12Å (1.16-1.12Å)  

R_all(%)

14.1 

R_work(%)

14.0 (18.9) 

R_free(%)

16.3 (19.9) 

Num. observed reflections

28142 (2519) 

Num. R_free reflections

1404 (119) 

Completeness(%)

97.7 (93.0) 

Model parameters

Num Atoms

1071  

Num Waters

131  

Num Hetatoms

173  

Model mean isotropic B factor

12.150Ų  

RMSD bond length

0.025Å  

RMSD bond angle

1.967°  

RMSD dihedral angle

12.055°

 

Filename uploaded

3TYS.pdb (uploaded on Oct 12, 2011 2:59 PM)  

Inserted

Jul 29, 2011