Structure of IDP90828
Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168
Edit deposit information
- CSGID target
- IDP90828
- PDB Id
- 3TPF (NCBI MMDB)
- Authors
- I.G.Shabalin,O.Onopriyenko,S.Grimshaw,P.J.Porebski,M.Grabowski,A.Savchenko,M.Chruszcz,W.F.Anderson,W.Minor,Center For Structural Genomics Of Infectious Diseases (Csgid)
- Responsible person
- Ivan Shabalin
- Responsible lab
- University of Virginia
- Deposition Date
- Sep 07, 2011
- Release Date
- Sep 21, 2011
Annotation
- Description
- Ornithine carbamoyltransferase (OTC) catalyzes the reversible transfer of the carbamoyl group from carbamoyl phosphate (CP) to the Nε atom of L-ornithine (ORN) to produce L-citrulline. There are two types of the enzyme – anabolic (aOTC) and catabolic (cOTC). Anabolic OTCes participate in the urea cycle and L-arginine biosynthesis. Catabolic OTCes are part of the catabolic arginine deiminase pathway found in a number of microorganisms. The reported structure is for the anabolic enzyme from food-borne pathogen Campylobacter jejuni. The structure has two trimers in the asymmetric unit.
- Functional assignment
Ligands
| Ligand code | Name | Ligand type |
|---|---|---|
| MSE | modified residue | |
| 175 | 3,5-dihydro-5-methylidene-4h-imidazol-4-on |
Structure information
Unit cell parameters
- Space Group
- P 21 21 2
- Unit Cell
-
a=157.16Å, b=87.40Å, c=141.56Å
α=90.00, β=90.00, γ=90.00 - Solvent content
- Matthews coefficient
- Resolution range
- 50.00-2.70Å (2.77-2.70Å)
- Rall(%)
- 20.4
- Rwork(%)
- 20.2 (34.9)
- Rfree(%)
- 24.0 (40.8)
- Num. observed reflections
- 54247 (3713)
- Num. Rfree reflections
- 2766 (170)
- Completeness(%)
- 99.8 (99.9)
- Num Atoms
- 13982
- Num Waters
- 104
- Num Hetatoms
- 0
- Model mean isotropic B factor
- 80.470Å2
- RMSD bond length
- 0.017Å
- RMSD bond angle
- 1.772°
- Filename uploaded
- hkl_refine_400_tls.pdb (uploaded on Sep 21, 2011 6:46 PM)
- Inserted
- Sep 21, 2011